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Monocytes were first stimulated with EBV for 20 hours, washed and then restimulated a second time with EBV for the indicated times. Epstein-Barr virus BARF1 protein is dispensable for B-cell transformation and inhibits alpha interferon secretion from mononuclear cells. Induction and regulation of IFNs during viral infections. However, in the case of phospho-IRF7, whilst levels of phosphorylation were detected after a second EBV stimulation, no further increase of phosphorylation levels was observed.

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